Description
Recombinant human MYO3beta (1-326) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The gene accession number is NM_138995.
MYO3beta is a member of the Class III myosins that are actin-dependent motor proteins containing an amino-terminal kinase domain. MYO3beta contains a N-terminal kinase domain, followed by motor, neck, and tail domains. The MYO3beta gene generates a variety of splice variants that contain 1 or 2 calmodulin-binding (IQ) motifs in the neck domain and 1 of 3 domains in the tail domain. Northern blot analysis shows expression of a 7-kb MYO3beta transcript in the human retina but not in a RPE cell line (1). The MYO3beta gene transcript is also detected in the kidney, intestine and testis. The MYO3beta gene maps to chromosome 2q31.1-q31.2 by genomic sequence alignment